![]() The separated protein from gel get transferred to nitrocellulose paper by capillary action. The nitrocellulose membrane is placed on the gel.Protein are negatively charged, so they move toward positive (anode) pole as electric current is applied.The small size protein moves faster than large size protein.The proteins are separated on the basis of electric charge, isoelectric point, molecular weight, or combination of these all. ![]()
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